Agilent 1290 Infinity II LC System

Welcome to the infinitely better liquid chromatography system. Innovative new technology delivers more resolution per time,more sensitivity and more flexibilityThe debate is over. The advantages of smaller particles—speed, resolution and sensitivity—were developed 40 years ago. The technology took a giant step forward in 2003 when Agilent introduced the first totally porous sub-2 μm particle columns, the ZORBAX Rapid Resolution High Throughput (RRHT) columns. The availability of other advanced particle columns provided additional ways to achieve further gains in separation power per time by using higher flow rates. Today, about a dozen vendors offer dedicated UHPLC systems. All of them operate at different pressures, flow rates, system volumes, temperatures and detector sensitivities. So each system suffers from performance limitations for specific column dimensions or particle technology. And methods transferability between hese systems is either impossible or requires redevelopment and revalidation. Here’s why the 1290 Infinity LC is the last word. With the Agilent 1290 Infinity LC, you’re no longer limited in your choice of column dimension, particle type, mobile and stationary phase, flow rate or pressure. Whatever the chromatography requires for optimum performance, the system can handle it, thanks to its unique power range and next-generation technology. This also includes the new ZORBAX Rapid Resolution High Definition (RRHD) 1.8 μm columns—the only columns for up to 1200 bar operation. The 1290 Infinity LC is also the first system to give you a foundation for methods transfer to or from any Agilent or non-Agilent UHPLC or HPLC system. In short, infinite capabilities are available to solve all your HPLC, UHPLC and LC/MS challenges.

Who can argue with that?

Infinitely more powerful Agilent’s new 1290 Infinity Binary Pump gives you maximum chromatographic performance, compatibility and flexibility.Active damping combines with lowest delay volumes—facilitated by propriety multi-layer technology in the new Agilent Jet Weaver mixer—to provide ultra fast gradients and superior LC/UV and LC/MS performance.

Infinitely more sensitive Featuring an innovative optical design—including Agilent Max-Light cartridge flow cells with optofluidic wave guides the new 1290 Infinity Diode Array Detector delivers a new level of UV sensitivity and baseline robustness. For LC/MS, Agilent Jet Stream Thermal Focusing technology significantly increases MS and MS/MS sensitivity by improving the spatial focusing of electro spray droplets. Infinitely more flexible New Agilent Quick-Change valves in the new 1290 Infinity Thermostatted Column Compartment enable resource-saving ultra high-throughput, multi-method and automated method  development solutions. A totally new module—the 1290 Infinity Flexible Cube allows fixed-loop injection mode for ultra fast cycle times, or automatic backflushing of the needle seat for a new benchmark in lowest carryover.


  • Maximize Analytical Efficiency More chromatographic resolution – specially designed components in the sample flow path achieve lowest system dispersion.
  • Higher peak capacity for challenging separations ­– switch easily between single dimension UHPLC and the ultimate chromatographic power of 2D-LC.
  • Lower carryover for uncompromised data quality – multiwash capabilities of the 1290 Infinity II Multisampler reduce carryover to less than 10 ppm even for challenging compounds such as chlorohexidine.
  • Unique detection capabilities - combine lowest detection limits with an ultra-wide dynamic range by using the new 1290 Infinity II HDR- DAD or 1290 Infinity II ELSD.
  • Maximize Instrument Efficiency Faster injection cycles with dual-needle injection and smart overlapped injections offer higher sample throughput
  • Higher sample capacity per bench space – up to 6144 samples within the footprint of a standard Agilent stack.
  • Significantly better usability - dead-volume-free UHPLC fluidic connections can be easily achieved by the revolutionary A-line quick-connect fittings.
  • Flexibility for all applications – due to the wide power, temperature and automatically scalable injection range, gradient options and intelligent system emulation technology.
  • Maximize Laboratory Efficiency Seamless transfer of methods between LCs, regardless of the brand – facilitated by Intelligent System Emulation Technology (ISET) delivering unchanged retention time and peak resolution.
  • Seamless integration in your chromatography data system – Agilent LC instrumentation runs smoother than ever before when controlled through third-party chromatography data systems such as Waters Empower or Thermo Scientific, thanks to the Agilent Instrument Control Framework (ICF).
  • Lower total cost of ownership– select from a variety of tools to achieve lowest cost-per-sample.
  • Software and Informatics